Publications

@article{nokey,

title = {Effect of G4C2-Repeat Expansions on the Motion of Lysosomes Inside Neurites. },

author = {Maria Mytiliniou and Joeri A.J. Wondergem and Marleen Feliksik and Thomas Schmidt and Thomas Fuhs and Doris Heinrich},

url = {https://doi.org/10.1088/1478-3975/ae5143},

doi = {10.1088/1478-3975/ae5143},

year  = {2026},

date = {2026-03-12},

urldate = {2026-03-12},

journal = {Physical Biology},

volume = {23},

pages = {026008},

abstract = {The G4C2 hexanucleotide repeat expansion in the c9orf72 locus is a mutation associated with amyotrophic lateral sclerosis. Recent evidence suggests a link with disrupted axonal trafficking in neurons. Here, using a neuronal-like cell line without or transfected with G4C2 repeats, we characterize the motion of lysosomes inside neurites. The neurites grew either aligned to patterned lines, or oriented freely on a 2D-substrate. Implementing time-resolved (local) mean squared displacement analysis lysosome trajectories were split into sub-diffusive, diffusive, and super-diffusive parts. Our results suggest that in the presence of the G4C2 repeats, lysosome trafficking is hampered, exhibiting overall decreased mean squared displacement and speed, more prominently inside aligned neurites. Moreover, a prominent effect in the super-diffusive drift velocity and diffusive motion diffusion coefficient was evident when the motion occurred inside aligned neurites. Trajectories which included super-diffusive motion, exhibited a varied ratio of anterograde/retrograde/neutral for both neurite geometries in the presence of G4C2 repeats but a similar velocity decrease for both directions in each neurite geometry. Our findings support the hypothesis that impaired axonal trafficking emerges in the presence of the G4C2 hexanucleotide repeat expansion, and demonstrate that this effect is more prominent when the neurites are aligned.},

howpublished = {bioRxiv 2021.10.29.466472},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2797,

title = {Deformable hydrospheres and cell segmentation report on stresses generated by individual cells in tumoroids},

author = {Rick Rodrigues de Mercado and Klara Beslmüller and Daan Vorselen and Erik H. J. Danen and Thomas Schmidt},

url = {https://doi.org/10.1016/j.xcrp.2025.103040},

doi = {10.1016/j.xcrp.2025.103040},

issn = {2666-3864},

year  = {2026},

date = {2026-01-02},

journal = {Cell Reports Physical Science},

volume = {7},

pages = {103040},

abstract = {The reciprocal interplay between cancer cells and their local environment, mediated by mechanical forces, requires quantitative measurements of cellular forces within the intricate three-dimensional context of the extracellular matrix. In the current study, we introduced an approach that utilizes soft, elastic hydrogel micro-particles, resembling the size of cells, to serve as specific and sensitive traction sensors in three-dimensional cell culture of collagen-embedded tumoroids. Our methodology relies on high-resolution detection of micro-particle deformations, which are subsequently translated into spatially resolved traction fields, reaching micrometer spatial resolution and simultaneously detecting traction forces as low as 30 Pa. Measurements enabled us to explore the relationships between cellular characteristics, extracellular traction fields, and cellular responses. We observed that cellular stresses ranged from 10 to 100 Pa, integrating cellular forces from 0.1 to 100 nN. The interaction of cells with inert microparticles appeared to be governed by contact mechanics resembling that of two soft spheres.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2787,

title = {Matrix Stiffness Affects Spheroid Invasion, Collagen Remodeling, and Effective Reach of Stress into ECM},

author = {Klara Beslmüller and Rick Rodrigues Mercado and Gijsje H. Koenderink and Erik H. J. Danen and Thomas Schmidt},

url = {https://www.mdpi.com/2674-1172/4/2/11},

issn = {2674-1172},

year  = {2025},

date = {2025-01-01},

journal = {Organoids},

volume = {4},

number = {2},

pages = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2786,

title = {Deciphering the allosteric control of androgen receptor DNA binding by its disordered N-terminal domain},

author = {Laurens W. H. J. Heling and Jolieke Veen and Adam Rofe and Eric West and Alba Jiménez-Panizo and Andrea Alegre-Martí and Vahid Sheikhhassani and Julian Ng and Thomas Schmidt and Eva Estébanez-Perpiñá and Iain J. McEwan and Alireza Mashaghi},

url = {https://www.sciencedirect.com/science/article/pii/S0303720725001856},

doi = {https://doi.org/10.1016/j.mce.2025.112634},

issn = {0303-7207},

year  = {2025},

date = {2025-01-01},

journal = {Molecular and Cellular Endocrinology},

volume = {608},

pages = {112634},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2723,

title = {Differential sensitivity to ionizing radiation in gemcitabine- and paclitaxel-resistant pancreatic cancer cells},

author = {Pei Pei Che and Alessandro Gregori and Cecilia Bergonzini and Mahsoem Ali and Giulia Mantini and Thomas Schmidt and Francesco Finamore and Stephanie M. Fraga Rodrigues and Adam E. Frampton and Liam A. McDonnell and Erik H. Danen and Ben J. Slotman and Peter Sminia and Elisa Giovannetti},

url = {https://doi.org/10.1016/j.ijrobp.2023.10.035},

doi = {10.1016/j.ijrobp.2023.10.035},

issn = {0360-3016},

year  = {2024},

date = {2024-12-01},

journal = {International Journal of Radiation Oncology, Biology, Physics},

volume = {118},

pages = {1328-1343},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2766,

title = {Altered Mechanobiology of PDAC Cells with Acquired Chemoresistance to Gemcitabine and Paclitaxel},

author = {Alessandro Gregori and Cecilia Bergonzini and Mjriam Capula and Rick Rodrigues Mercado and Erik H. J. Danen and Elisa Giovannetti and Thomas Schmidt},

url = {https://www.mdpi.com/2072-6694/16/22/3863},

issn = {2072-6694},

year  = {2024},

date = {2024-01-01},

journal = {Cancers},

volume = {16},

number = {22},

pages = {3863},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2764,

title = {ABCB1 overexpression through locus amplification represents an actionable target to combat paclitaxel resistance in pancreatic cancer cells},

author = {Cecilia Bergonzini and Alessandro Gregori and Tessa M. S. Hagens and Vera E. Noord and Bob Water and Annelien J. M. Zweemer and Bircan Coban and Mjriam Capula and Giulia Mantini and Asia Botto and Francesco Finamore and Ingrid Garajova and Liam A. McDonnell and Thomas Schmidt and Elisa Giovannetti and Erik H. J. Danen},

url = {https://doi.org/10.1186/s13046-023-02879-8},

doi = {10.1186/s13046-023-02879-8},

issn = {1756-9966},

year  = {2024},

date = {2024-01-01},

journal = {Journal of Experimental & Clinical Cancer Research},

volume = {43},

number = {1},

pages = {4},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2750,

title = {CD3-engaging bispecific antibodies trigger a paracrine regulated wave of T-cell recruitment for effective tumor killing},

author = {Chen-Yi Liao and Patrick Engelberts and Andreea Ioan-Facsinay and Janna Eleonora Klip and Thomas Schmidt and Rob Ruijtenbeek and Erik H. J. Danen},

url = {https://doi.org/10.1038/s42003-024-06682-9},

doi = {10.1038/s42003-024-06682-9},

issn = {2399-3642},

year  = {2024},

date = {2024-01-01},

journal = {Communications Biology},

volume = {7},

number = {1},

pages = {983},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2708,

title = {Hexanematic crossover in epithelial monolayers depends on cell adhesion and cell density},

author = {Julia Eckert and Benoît Ladoux and René-Marc Mège and Luca Giomi and Thomas Schmidt},

url = {https://doi.org/10.1038/s41467-023-41449-6},

doi = {10.1038/s41467-023-41449-6},

issn = {2041-1723},

year  = {2023},

date = {2023-01-01},

journal = {Nature Communications},

volume = {14},

number = {1},

pages = {5762},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2791,

title = {Substrate rigidity modulates traction forces and stoichiometry of cell-matrix adhesions},

author = {H. E. Balcioglu and R. Harkes and E. H. J. Danen and T. Schmidt},

doi = {10.1063/5.0077004},

issn = {0021-9606},

year  = {2022},

date = {2022-01-01},

journal = {J Chem Phys},

volume = {156},

number = {8},

pages = {085101},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2632,

title = {Holding it together: when cadherin meets cadherin},

author = {F. N. Arslan and J. Eckert and T. Schmidt and C. P. Heisenberg},

doi = {10.1016/j.bpj.2021.03.025},

issn = {0006-3495},

year  = {2021},

date = {2021-01-01},

journal = {Biophys J},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2631,

title = {Hypergravity affects cell traction forces of fibroblasts},

author = {J. Eckert and Jjwa Loon and L. M. Eng and T. Schmidt},

doi = {10.1016/j.bpj.2021.01.021},

issn = {0006-3495 (Print) 

0006-3495},

year  = {2021},

date = {2021-01-01},

journal = {Biophys J},

volume = {120},

number = {5},

pages = {773-780},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2666,

title = {Fibronectin Patches as Anchoring Points for Force Sensing and Transmission in Human Induced Pluripotent Stem Cell-Derived Pericytes},

author = {O. Iendaltseva and V. V. Orlova and C. L. Mummery and E. H. J. Danen and T. Schmidt},

url = {https://www.ncbi.nlm.nih.gov/pubmed/32470326},

doi = {10.1016/j.stemcr.2020.05.001},

issn = {2213-6711 (Electronic) 

2213-6711 (Linking)},

year  = {2020},

date = {2020-01-01},

journal = {Stem Cell Reports},

volume = {14},

number = {6},

pages = {1107-1122},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN2630,

title = {Mechanical interplay between cell shape and actin cytoskeleton organization},

author = {Koen Schakenraad and Jeremy Ernst and Wim Pomp and Erik H. J. Danen and Roeland M. H. Merks and Thomas Schmidt and Luca Giomi},

url = {http://dx.doi.org/10.1039/D0SM00492H},

doi = {10.1039/D0SM00492H},

issn = {1744-683X},

year  = {2020},

date = {2020-01-01},

journal = {Soft Matter},

volume = {16},

number = {27},

pages = {6328-6343},

keywords = {},

pubstate = {published},

tppubtype = {article}

}